AIF1/Iba1 Rabbit Polyclonal Antibody

• Catalog Number: orb10863
• Category: Antibodies
• Description: AIF1/Iba1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Iba1 (51-147/147aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 16 kDa
• Target: AIF1
• UniProt ID: P55008
• RRID: AB_10751100
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AIF 1; AIF1; AIF1 protein; IBa1; iba-1; IBa 1; all
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Balbinot, Camille et al. The Cdx2 homeobox gene suppresses intestinal tumorigenesis through non-cell-autonomous mechanisms J Exp Med, 215, 911-926 (2018)

PubMed: 29439001

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AIF1) Polyclonal Antibody, Unconjugated (orb10863) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Mouse) lysate at 30 ug, Primary:Anti-AIF1/Iba1 (orb10863) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 16 kD, Observed band size: 16 kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-AIF1/IBa-1 Polyclonal Antibody, Unconjugated (orb10863) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

U-937 cells were fixed with 4% PFA for 10 min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AIF1 Antibody (orb10863) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).