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AIF1/Iba1 Rabbit Polyclonal Antibody (FITC)

Catalog Number: orb15805

DispatchUsually dispatched within 2-3 weeks
$ 450.00
Catalog Numberorb15805
CategoryAntibodies
DescriptionAIF1/Iba1 Rabbit Polyclonal Antibody (FITC)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Iba1 (51-147/147aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIF=1:100-500
ConjugationFITC
TargetAIF1
RRIDAB_10746337
StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesAIF 1; AIF1; AIF1 protein; IBa1; iba-1; IBa 1; all
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NoteFor research use only
Expiration Date12 months from date of receipt.
AIF1/Iba1 Rabbit Polyclonal Antibody (FITC)

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-AIF1/Iba1 antibody (orb15805), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

AIF1/Iba1 Rabbit Polyclonal Antibody (FITC)

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-AIF1/Iba1 antibody (orb15805), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

AIF1/Iba1 Rabbit Polyclonal Antibody (FITC)

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-AIF1/Iba1 antibody (orb15805), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

AIF1/Iba1 Rabbit Polyclonal Antibody (FITC)

Blank control: U937. Primary Antibody (green line): Rabbit Anti-AIF1/Iba1 antibody (orb15805), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.