AIF1/IBA1 isoform 1 and 3 Antibody

• Catalog Number: orb18542
• Category: Antibodies
• Description: Goat polyclonal antibody to Interferon gamma
• Target: AIF1/IBA1 isoform 1 and 3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TGPPAKKAISELP
• RRID: AB_10750970
• MW: 10.5; 16.7
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.5-1μg/ml, IHC-P: 5μg/ml
• Application notes: ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Mouse Brain shows distinct staining of astrocytes. Recommended concentration, 2-4µg/ml. Data obtained from a previous batch (different goat).WB: Approx 17kDa band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 0.1-0.3µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AIF1; IBLY409840A1; allograft inflammatory factor
• Research Area: Cancer, Epigenetics, Neuroscience
• Note: For research use only
• Entrez: 199
• Expiration Date: 12 months from date of receipt.

References

Song-I Seol 1, Sang-A Oh 1, Dashdulam Davaanyam 1, Ja-Kyeong Lee Blocking peptidyl arginine deiminase 4 confers neuroprotective effect in the post-ischemic brain through both NETosis-dependent and -independent mechanisms Acta Neuropathol Commun, (2025)

PubMed: 39966968

Applications

IF

Reactivity

Rat

1 µg/ml staining of Mouse (A) and (3 µg/ml) Rat (B) Brain lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

6 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Negative Control showing staining of paraffin embedded Human Frontal Cortex, with no primary antibody.

4 µg/ml staining of paraffin embedded Human Frontal Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.