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Goat anti-AIF1 / IBA1 (isoform 3) Antibody

Catalog Number: orb19198

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb19198
CategoryAntibodies
DescriptionGoat polyclonal antibody to Interferon gamma
TargetAIF1 / IBA1 (isoform 3)
ClonalityPolyclonal
Species/HostGoat
ConjugationUnconjugated
ReactivityCanine, Human, Mouse
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Protein SequenceNKQFLDDPKYSSDED
RRIDAB_10755905
MW16.7
Tested applicationsELISA, FC, IF, IHC
Dilution rangeELISA: 1:32000, WB: 0.5-1.5 μg/ml, FACS: 10ug/ml, IF/ICC: 10μg/ml
Application notesIHC: Paraffin embedded Human Lung and Brain (Cortex). Recommended concentration: 5-6µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membrane of U937, Caco-2 and U251 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAIF1, IBA1, allograft inflammatory factor 1 , DASS
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NoteFor research use only
Entrez199
Goat anti-AIF1 / IBA1 (isoform 3) Antibody

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Goat anti-AIF1 / IBA1 (isoform 3) Antibody

7 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Goat anti-AIF1 / IBA1 (isoform 3) Antibody

Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Goat anti-AIF1 / IBA1 (isoform 3) Antibody

5 µg/ml staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Goat anti-AIF1 / IBA1 (isoform 3) Antibody

Immunofluorescence analysis of paraformaldehyde fixed U937 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Goat anti-AIF1 / IBA1 (isoform 3) Antibody

Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Goat anti-AIF1 / IBA1 (isoform 3) Antibody

Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.