AIF1/IBA1 (isoform 3) Antibody

SKU: orb19198

Description

Goat polyclonal antibody to Interferon gamma

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Dilution range: ELISA: 1:32000, WB: 0.5-1.5 μg/ml, FACS: 10ug/ml, IF/ICC: 10μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse
• Application Notes:
  IHC: Paraffin embedded Human Lung and Brain (Cortex). Recommended concentration: 5-6µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membrane of U937, Caco-2 and U251 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: AIF1 / IBA1 (isoform 3)
• Molecular Weight: 16.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

AIF1, IBA1, allograft inflammatory factor 1 , DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

7 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

5 µg/ml staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Immunofluorescence analysis of paraformaldehyde fixed U937 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

Quick Database Links

Gene Symbol

AIF1 / IBA1 (isoform 3)

NCBI Gene ID

199