Goat anti-AIF1 / IBA1 (isoform 3) Antibody

• Catalog Number: orb19198
• Category: Antibodies
• Description: Goat polyclonal antibody to Interferon gamma
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Canine, Human, Mouse
• Dilution range: ELISA: 1:32000, WB: 0.5-1.5 μg/ml, FACS: 10ug/ml, IF/ICC: 10μg/ml
• Conjugation: Unconjugated
• MW: 16.7
• Target: AIF1 / IBA1 (isoform 3)
• Entrez: 199
• Protein Sequence: NKQFLDDPKYSSDED
• RRID: AB_10755905
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: AIF1, IBA1, allograft inflammatory factor 1 , DASS
• Note: For research use only
• Application notes: IHC: Paraffin embedded Human Lung and Brain (Cortex). Recommended concentration: 5-6µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membrane of U937, Caco-2 and U251 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human frontal cortex lysate (35ug protein in RIPA buffer) using Interferon gamma antibody

Flow Cytometry analysis of K562 cells tissue using Interferon gamma antibody

Immunofluorescence analysis of Caco-2 cells of Interferon gamma antibody