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Catalog Number | orb19198 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to Interferon gamma |
Target | AIF1 / IBA1 (isoform 3) |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Canine, Human, Mouse |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | NKQFLDDPKYSSDED |
RRID | AB_10755905 |
MW | 16.7 |
Tested applications | ELISA, FC, IF, IHC |
Dilution range | ELISA: 1:32000, WB: 0.5-1.5 μg/ml, FACS: 10ug/ml, IF/ICC: 10μg/ml |
Application notes | IHC: Paraffin embedded Human Lung and Brain (Cortex). Recommended concentration: 5-6µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membrane of U937, Caco-2 and U251 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | AIF1, IBA1, allograft inflammatory factor 1 , DASS Read more... |
Note | For research use only |
Entrez | 199 |
Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
7 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
5 µg/ml staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.
Immunofluorescence analysis of paraformaldehyde fixed U937 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.