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ZO-1 Antibody

SKU: orb20387

Description

Goat polyclonal to ZO-1

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IF
Dilution RangeELISA: 1:8000, IF/ICC: 10 μg/ml
ReactivityHuman
Application Notes
This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2)ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 170kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 187kDa according to NP_783297.2). Recommended concentration: 0.3-1 μg/ml.

Key Properties

ClonalityPolyclonal
TargetZO-1
Protein SequencePKPTSQNQFSEHDKT
Molecular Weight195; 187
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti TJP1 antibody, anti tight junction protein 1 (zona occludens 1) antibody, anti DKFZp686M05161 antibody, anti MGC133289 antibody, anti ZO-1 antibody, anti OTTHUMP00000174520 antibody, anti tight junction protein 1 antibody, anti tight junction protein ZO-1 antibody, anti zona occludens 1 antibody, anti zonula occludens 1 protein antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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