ZO-1 Antibody

SKU: orb20387

Description

Goat polyclonal to ZO-1

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IF
• Dilution Range: ELISA: 1:8000, IF/ICC: 10 μg/ml
• Reactivity: Human
• Application Notes:
  This antibody is expected to recognize both reported isoforms (NP_003248.3; NP_783297.2)ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 170kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 187kDa according to NP_783297.2). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ZO-1
• Protein Sequence: PKPTSQNQFSEHDKT
• Molecular Weight: 195; 187
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TJP1 antibody, anti tight junction protein 1 (zona occludens 1) antibody, anti DKFZp686M05161 antibody, anti MGC133289 antibody, anti ZO-1 antibody, anti OTTHUMP00000174520 antibody, anti tight junction protein 1 antibody, anti tight junction protein ZO-1 antibody, anti zona occludens 1 antibody, anti zonula occludens 1 protein antibody

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cellular junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue) Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Quick Database Links

Gene Symbol

ZO-1

NCBI Gene ID

7082