ZO-1 antibody

• Catalog Number: orb11587
• Category: Antibodies
• Description: ZO-1 antibody
• Target: ZO-1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Immunogen: KLH conjugated synthetic peptide derived from human ZO-1. Please contact us for the exact immunogen sequence. The peptide is available as orb374898.
• UniProt ID: Q07157, P39447
• RRID: AB_10751121
• MW: 191 kDa
• Tested applications: ELISA, FACS, IHC-P
• Dilution range: P-ELISA: 1:500-1000 (based on 0.5 mg/ml)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-DKFZp686M05161 antibody, anti-MGC133289 antib
• Note: For research use only
• Entrez: 7082
• NCBI: 003257, 31, 003248

References

Woolf, Eric C. et al. The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model PLoS One, 10, e0130357 (2015)

PubMed: 26083629

Applications

WB

Reactivity

Mouse

Zhao, Yuan et al. Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets Int J Mol Sci, 12, 8502-8512 (2011)

PubMed: 22272087

Applications

IHC

Reactivity

Porcine

Lili Liu , Xiaoyi Gong , Xinyu Zhang , Dawei Zhang , Ying Tang ,Jiantao Liu , Yajie Li , Disheng Pan Resveratrol Alleviates Heat-Stress-Induced Impairment of the Jejunal Mucosa Through TLR4/MAPK Signaling Pathway in Black-Boned Chicken Poultry Science, 103242 (2023)

PubMed: 37980746

Applications

IHC

Reactivity

Gallus

Anna Arczewska-Wlosek,Sylwester Swiatkiewicz,Ewa Tomaszewska,Siemowit Muszynski,Piotr Dobrowolski and Damian Jozefiak Effects of Anticoccidial Vaccination and Taraxacum officinale Extract on the Growth Performance, Biochemical Parameters, Immunity, and Intestinal Morphology of Eimeria-Challenged Chickens MDPI, 13(9), 1927 (2023)

PubMed: 37763330

Applications

IHC

Reactivity

Gallus

Immunohistochemical staining of mouse colon stained tissue using ZO-1 antibody

Flow cytometric analysis of HepG 2 cells using ZO-1 antibody

Immunohistochemical staining of human testis tissue using ZO-1 antibody

Flow cytometric analysis of 293T Cells using ZO-1 antibody

Immunohistochemical staining of human placenta tissue using ZO-1 antibody

Immunohistochemical analysis of paraffin-embedded rat brain tissue using ZO-1 antibody