ZO-1 antibody

SKU: orb313868

Description

ZO-1 antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-P
• Dilution range: IF/ICC: 1:100-500, IHC-P: 1:100-500

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ZO-1. Please contact us for the exact immunogen sequence. The peptide is available as orb374960.
• Target: ZO-1
• Molecular Weight: 191 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti-DKFZp686M05161 antibody, anti-MGC133289 antibody, anti-Tight junction protein 1 antibody, anti-Tight junction protein ZO1 antibody, anti-TJP1 antibody, anti-ZO-1 antibody, anti-Zona occludens 1 protein antibody, anti-Zonula occludens 1 protein antibody

Immunohistochemical staining of mouse lung tissue using anti-ZO-1 (dilution of primary antibody - 2.5 ug/ml)

IHC-P image of rat muscle tissue using ZO-1 antibody (dilution of primary antibody at 2.5 ug/ml)

IHC-P staining of rat lymph node tissue using anti-ZO-1 (dilution at 2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat colon tissue using anti-ZO-1 (primary antibody at 2.5 ug/ml)

Immunofluorescence image of human hela cells tissue using ZO-1 antibody (dilution at 2.5 ug/ml)

Immunohistochemical staining of rat lymph node tissue using ZO-1 antibody (dilution of primary antibody - 2.5 ug/ml)

Immunohistochemical staining of rat colon tissue using anti-ZO-1 (dilution of primary antibody - 2.5 ug/ml)

IF image of human u251 cells tissue using anti-ZO-1 (primary antibody at 2.5 ug/ml)

Immunofluorescence analysis of human hela cells tissue using ZO-1 antibody (dilution of primary antibody - 2.5 ug/ml)

Immunofluorescence image of human u251 cells tissue using ZO-1 antibody (dilution at 2.5 ug/ml)

IHC-P image of mouse lung tissue using anti-ZO-1 (dilution of primary antibody at 2.5 ug/ml)

Quick Database Links

Gene Symbol

ZO-1

UniProt

Q07157, Q8R4T5, P39447

NCBI Gene ID

7082

RefSeq:003248, 003257

References

Elham Zahedi 1 2, Fatemeh Mokhtari Naseri 3, Elham Zamani 4, Marjan Nikbakhtzadeh 5, Tayebeh Rastegar 6, Ashkan Sanaeirad 7, Seyed Shahabeddin Sadr Ginger Extract Improves Cognitive Dysfunction via Modulation of Gut Microbiota-Derived Short-Chain Fatty Acids in D-Galactose/Ovariectomy-Induced Alzheimer-Like Disease Mol Neurobiol ., (2024)

PubMed: 39505806

Puzio, Iwona et al. Alterations in Small Intestine and Liver Morphology, Immunolocalization of Leptin, Ghrelin and Nesfatin-1 as Well as Immunoexpression of Tight Junction Proteins in Intestinal Mucosa after Gastrectomy in Rat Model J Clin Med, 10, E272 (2021)

PubMed: 33450994