TRIM21 Antibody

• Catalog Number: orb1238609
• Category: Antibodies
• Description: TRIM21 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-TRIM21 antibody (orb1238609) was raised against a peptide corresponding to 16 amino acids near the center of human TRIM21. The immunogen is located within amino acids 290-340 of TRIM21.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 54kDObserved: 54 kD
• Target: TRIM21
• UniProt ID: P19474
• NCBI: NP_003132
• Storage: TRIM21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: TRIM21 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: TRIM21 Antibody: SSA, RO52, SSA1, RNF81, Ro/SSA, E
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Lung Tissue Lysate in (Lane 1) the absence and (Lane 2) the presence of blocking peptide. Loading: 15 µg of lysates per lane. Antibodies: TRIM21, orb1238609 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TRIM21, orb1238609 (0.5 µg/mL), TRIM21, orb1258368 (2 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of TRIM21 in Human Small Intestine Tissue. Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TRIM21 antibody (orb1238609) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of TRIM21 in Human Small Intestine Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human small intestine tissue labeling TRIM21 with orb1238609 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).