Goat anti-SSA1 / Ro52 Antibody

• Catalog Number: orb18491
• Category: Antibodies
• Description: Goat polyclonal antibody to TRIM21
• Target: SSA1 / Ro52
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CPLNIGSQGSTDY
• RRID: AB_10751227
• MW: 54.2
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:16000, WB: 0.2-1 μg/ml, IHC-P: 10ug/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 38kDa and 25kDa in Human Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 54.2kDa according to NP_003132.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TRIM21 antibody, anti tripartite motif-contai
• Note: For research use only
• Entrez: 6737

Western blot analysis of HEK293 lysate using TRIM21 antibody

Immunohistochemical staining of Human Colon using TRIM21 antibody

Immunofluorescence analysis of U2OS cells using TRIM21 antibody

Flow cytometric analysis of HeLa cells using TRIM21 antibody