TRIM21/RO52 Antibody

SKU: orb1537830

Description

TRIM21/RO52 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: IF (10 µg/ml), IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:16000), WB (0.2 - 1 µg/ml)
• Reactivity: Human, Monkey
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence CPLNIGSQGSTDY, from the C-Terminus of protein sequence according to NP_003132.2.
• Target: TRIM21 / RO52
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

TRIM21, 52 kDa Ro protein, RING finger protein 81, RO52, Ro(SS-A), SSA1, Tripartite motif-containing 21, RNF81, SSA, Sicca syndrome antigen A, SS-A, Tripartite motif containing 21

TRIM21 / RO52 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with TRIM21 / RO52 antibody (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C.

TRIM21 / RO52 antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton.

Quick Database Links

Gene Symbol

TRIM21 / RO52