TRIM21/RO52 Antibody

• Catalog Number: orb1537830
• Category: Antibodies
• Description: TRIM21/RO52 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Reactivity: Human, Monkey
• Immunogen: Peptide with sequence CPLNIGSQGSTDY, from the C-Terminus of protein sequence according to NP_003132.2.
• Concentration: 0.5 mg/ml
• Dilution range: IF (10 µg/ml), IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:16000), WB (0.2 - 1 µg/ml)
• Conjugation: Unconjugated
• Target: TRIM21 / RO52
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: TRIM21, 52 kDa Ro protein, RING finger protein 81,
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B2588 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2588 was determined to be 5 ug/ml.
• Expiration Date: 12 months from date of receipt.

TRIM21 / RO52 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with TRIM21 / RO52 antibody (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C.

TRIM21 / RO52 antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton.