TRIM21 Antibody

SKU: orb1247003

Description

TRIM21 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.2-1µg/mL. Primary incubations were for 1 hour.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10µg/mL. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CPLNIGSQGSTDY
• Target: TRIM21
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TRIM21, tripartite motif-containing 21, RNF81, RO52, SSA, SSA1, 52kD Ro/SSA autoantigen, 52kDa ribonucleoprotein autoantigen SS-A/Ro, Sicca syndrome antigen A, Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro), Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro), tripartite motif protein TRIM21, tripartite motif protein 21

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with orb1247003 (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with orb1247003 (1 mg/ml) in Lane B.

orb1247003 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247003 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing strong nuclear and some cytoplasmic staining.

orb1247003 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

TRIM21

UniProt

P19474

RefSeq (Protein):NP_003132.2