TNF alpha Rabbit Polyclonal Antibody

• Catalog Number: orb100003
• Category: Antibodies
• Description: TNF alpha Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Canine, Equine, Mouse, Porcine
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TNF-alpha (181-235/233aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17/26 kDa
• Target: TNF
• UniProt ID: P01375
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Tumor necrosis factor-α; TNFalpha; APC1; Cachectin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-TNF alpha (orb100003) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 26/17 kD, Observed band size: 26 kD.

Tissue/cell: human pneumonia tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-TNF-alpha Polyclonal Antibody, Unconjugated (orb100003) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.