TNF alpha Rabbit Polyclonal Antibody

SKU: orb371962

Description

TNF alpha Rabbit Polyclonal Antibody is an unconjugated antibody that specifically recognizes TNF alpha. Generated against a KLH conjugated synthetic peptide derived from human TNF alpha. The peptide is available as orb374984. It is supplied in liquid form and exhibits reactivity with Human, Mouse and Rat samples. The suitable applications for this antibody are ICC, IF, IHC-P.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ICC, IF, IHC-P
• Dilution Range: IF/ICC: 1:100-800, WB: 1:100-2000, IHC-P: 1:100-800 (based on 0.5 mg/ml)
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TNF alpha. Please contact us for the exact immunogen sequence. The peptide is available as orb374984.
• Target: TNF alpha
• Molecular Weight: 27 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-APC1 antibody, anti-Cachectin antibody, anti-DIF antibody, anti-Differentiation inducing factor antibody, anti-MCF antibody, anti-Necrosin antibody, anti-TNF a antibody, anti-TNF alpha antibody, anti-TNF antibody, anti-TNF Monocyte Derived antibody, anti-TNF-a antibody, anti-TNF-alpha antibody, anti-TNFA antibody, anti-Tumor necrosis factor alpha antibody, anti-TNF-alpha antibody

Quick Database Links

Gene Symbol

TNF alpha

UniProt

P16599, P06804, P01375

NCBI Gene ID

7124

RefSeq:000594, 000585

Mehmet Berker 1, Sibel Köktürk 1, Sibel Doğan 1, Emel Usta Myricetin ameliorates the effects of hydrogen peroxide-induced oxidative stress in human mesenchymal stem cells: an ultrastructural and immunocytochemical study Ultrastruct Pathol, (2025)

PubMed: 40247639

Applications

IHC

Reactivity

Human

Mahshid Kharaziha 1 2, Sahar Salehi 2, Mahshid Shokri 1, Seyed Mohsen Ahmadi Tafti 3 4, Thomas Scheibel 2 5 6 7 8 ROS-Scavenging Multifunctional Microneedle Patch Facilitating Wound Healing Adv Healthc Mater, (2025)

PubMed: 40832795

Applications

IHC

Reactivity

Human

H Keyhan, H Morovvati, MK Koohi, J Hassan, M Mazidi Hematological and Histological Changes in the Knee Cartilage of Male Rats Received Intra-Articular Injection of Bee Venom and Melittin journals.ut.ac.ir, (2025)

PubMed: