TNF alpha Rabbit Polyclonal Antibody

• Catalog Number: orb7100
• Category: Antibodies
• Description: TNF alpha Rabbit Polyclonal Antibody
• Target: TNF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human TNF-alpha (181-235/235aa)
• UniProt ID: P01375
• RRID: AB_10929448
• MW: 17/26 kDa
• Tested applications: FC, ICC
• Dilution range: Flow-Cyt=1ug/Test, ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Tumor necrosis factor-α; TNFalpha; APC1; Cachectin
• Research Area: Cancer Research, Growth Factors, Immunology, Infla
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Oh, Ha Na et al. Effects of Quercetin in a Mouse Model of Experimental Dry Eye Cornea, 34, 1130-1136 (2015)

PubMed: 26203745

Applications

IHC

Reactivity

Mouse

Correia, Marcelo Henrique et al. Immune response and Raman scattering assessment in rats skin after contact with Fusarium oxysporum metabolites Spectrochim Acta A Mol Biomol Spectrosc, 234, 118246 (2020)

PubMed: 32179464

Applications

IHC

Reactivity

Rat

Shi, Xiaowei et al. Panax notoginseng saponins administration modulates pro- /anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats Metab Brain Dis, 32, 221-233 (2017)

PubMed: 27585466

Applications

IHC, WB

Reactivity

Rat

Sample: Diseased lung (mouse) at 30 ug, Primary: Anti-TNF alpha (orb7100) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000 dilution, Predicted band size: 17/28 kD, Observed band size: 17/28 kD.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-TNF alpha (orb7100) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36/17/26 kD, Observed band size: 36 kD.

Sample: Liver (mouse) Lysate at 40 ug, Primary: Anti-TNF alpha (orb7100) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17/26kD, Observed band size: 25 kD.