TNF alpha Rabbit Polyclonal Antibody

SKU: orb1294347

Description

Rabbit polyclonal antibody to TNF alpha

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:500-1:1500, Immunofluorescence 1:200-1:300, Immunohistochemistry (Paraffin) 1:100-1:200
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Recombinant fragment corresponding to Human TNF alpha.
• Target: TNF alpha
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

anti-TNF alpha antibody at 1/700 dilution, Lysates at 50 µg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294347 overnight at 4°C.

anti-TNF alpha antibody at 1/700 dilution, Lysates at 50 µg per lane, This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294347 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294347 anti-TNF alpha antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human Liver cancer tissue labeling TNF alpha with orb1294347 at 1/150.

Quick Database Links

Gene Symbol

TNF alpha