THRA Antibody

• Catalog Number: orb1246862
• Category: Antibodies
• Description: THRA Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KEEMIRSLQQRP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60+50kDa bands observed in Human Bone Marrow lysates (calculated MW of 54.8kDa according to NP_003241.2 and of 46.8kDa according to NP_955366.1). Recommended concentration: 0.5-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Breast. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: THRA
• UniProt ID: P10827
• NCBI: NP_955366.1, NP_003241.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: THRA, thyroid hormone receptor, alpha (erythroblas
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60+50kDa bands observed in Human Bone Marrow lysates (calculated MW of 54.8kDa according to NP_003241.2 and of 46.8kDa according to NP_955366.1). Recommended concentration: 0.5-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Breast. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246862 (0.5 ug/ml) staining of Human Bone Marrow lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246862 (3.75 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246862 (3.75 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.