Thyroid Hormone Receptor Antibody

• Catalog Number: orb612656
• Category: Antibodies
• Description: Mouse monoclonal antibody to NR1D1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: H43
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2a
• Immunogen: Synthetic peptide from the full length Human Thyroid hormone receptor protein
• Concentration: 1mg/ml
• Dilution range: WB (1:500); IHC (1:100)
• Conjugation: Unconjugated
• MW: 70 kDa
• Target: Thyroid Hormone Receptor
• Entrez: 7067
• UniProt ID: P10827
• NCBI: NM_001190918.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: ERBA ALPHA antibody, ERBA beta antibody, ERBA1 ant
• Note: For research use only
• Application notes: A 1:500 dilution of SMC-561 was sufficient for detection of Pan-Thyroid hormone receptor in 10 ug of Hep G2 Human Hepatoblastoma Cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 7.5X.

Immunohistochemistry analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 4X.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) Thyroid Hormone Receptor Antibody. (D) Hoechst (E) Composite.

Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of Thyroid Hormone Receptor protein using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Load: 10 ug. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band (s): Higher molceular weight bands could be due to PTMs.