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Thyroid hormone receptor alpha Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB=1:500-2000, Flow-Cyt=1ug/Test |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Thyroid hormone receptor alpha (81-180/490aa) |
| Target | THRA |
| Molecular Weight | 55 kDa |
| Purification | Affinity purified by Protein A |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
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Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Thyroid hormone receptor alpha antibody (orb100337), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-Thyroid hormone receptor alpha (orb100337) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.

Sample: Liver (Rat) Lysate at 40 ug, Primary: Anti-Thyroid hormone receptor alpha (orb100337) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kD, Observed band size: 55 kD.
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Thyroid hormone receptor alpha Rabbit Polyclonal Antibody (orb100337)
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