SPG20 Antibody

• Catalog Number: orb1262401
• Category: Antibodies
• Description: SPG20 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine, Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This SPG20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-45 amino acids from the N-terminal region of human SPG20.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 73 kDa
• Target: SPG20
• UniProt ID: Q8N0X7
• NCBI: Q8N0X7
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Spartin, Spastic paraplegia 20 protein, Trans-acti
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SPG20 Antibody in NCI-H460 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain tissue reacted with SPG20 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.