Goat anti-SPARTIN Antibody

• Catalog Number: orb20437
• Category: Antibodies
• Description: Goat polyclonal antibody to SPG20
• Target: SPARTIN
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EASGTDVKQLDQGNK
• RRID: AB_10748041
• MW: 72.8
• Tested applications: ELISA, IF, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments in Human Brain (Cerebral and Frontal Cortex, Hippocampus) lysates gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SPG20 antibody, anti spastic paraplegia 20 (T
• Research Area: Epigenetics, Neuroscience
• Note: For research use only
• Entrez: 23111

Immunofluorescence analysis of using SPG20 antibody

Western blot analysis of HeLa lysate using SPG20 antibody