SPG20 Antibody (N-term)

• Catalog Number: orb39730
• Category: Antibodies
• Description: Peptide affinity purified rabbit polyclonal antibody.
• Target: SPART
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: Synthetic Peptide
• UniProt ID: Q8N0X7
• MW: 72833
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
• Antibody Type: Primary Antibody
• Clone Number: RB22531
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-Spastic paraplegia 20 protein antibody, anti-
• Research Area: Infectious Disease & Virology, Protein Biochemistr
• Note: For research use only
• NCBI: NP_001135767.1, NP_055902.1, NP_001135766.1, NP_001135768.1

Western blot analysis of SPG20 Antibody (N-term) in NCI-H460 cell line lysates (35 ug/lane). SPG20 (arrow) was detected using the purified Pab.

SPG20 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with SPG20 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.