SPARTIN Antibody

• Catalog Number: orb1249374
• Category: Antibodies
• Description: SPARTIN Antibody
• Target: SPG20
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-EASGTDVKQLDQGNK
• UniProt ID: Q8N0X7
• Tested applications: ELISA, IF, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in lysates of cell line HeLa (calculated MW of 72.8kDa according to NP_055902.1). Recommended concentration: 1-3ug/ml.Immunofluorescence: HeLa show dimished signals after knock down of expresssion by introduction of siRNA.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SPG20, spastic paraplegia 20 (Troyer syndrome), KI
• Note: For research use only
• NCBI: NP_055902.1

orb1249374 (2 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249374 (2 ug/ml) staining of methanol-fixed HeLa before (left) and after (right) introduction of siRNA.