SARS-CoV-2 (COVID-19) Spike S2 Antibody (IN)

• Catalog Number: orb1274036
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike S2 Antibody (IN)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Synthetic peptide located within the center of SARS-CoV-2 (COVID-19) Spike S1.
• Concentration: batch dependent
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• NCBI: QHD43416.1
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 10% glycerol, pH 7.2
• Alternative names: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody: S
• Note: For research use only
• Application notes: WB: 1:500-1000
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with Recombinant Protein. Loading: 1 ug of recombinant protein per lane. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, orb1274036, 1:500. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution.

ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein, orb1231624. Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, orb1274036. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Develop: 15 min, 100 uL/well. Stop: Stop buffer 50 uL/well.