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Catalog Number | orb668899 |
---|---|
Category | Proteins |
Description | It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Reactivity | Virus |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 34.9 kDa |
Target | Spike NTD |
Protein Sequence | QHD43416.1 |
Source | SARS-CoV-2 S1 protein NTD, His Tag (orb668899) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1). |
Expression System | HEK293 |
Expression Region | Ser 13 - Leu 303 |
Endotoxins | 1.0 EU per μg |
NCBI | QHD43416.1 |
Storage | -20°C |
Buffer/Preservatives | PBS,pH7.3 |
Alternative names | S1 protein NTD,Spike protein S1 NTD,BetaCoV S1-NTD Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 34.9 kDa. The protein migrates as 45-65 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 33.7 kDa after removal of the signal peptide. The apparent molecular mass of S1-NTD-His is approximately 55 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 59.1 kDa after removal of the signal peptide.The apparent molecular mass of S1-NTD-mFc is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 59.0 kDa after removal of the signal peptide. The apparent molecular mass of S1-NTD-hFc is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
ELISA, WB | |
> 90% as determined by SDS-PAGE. | |
34.9 kDa | |
HEK293 cells |
≥92% as determined by SDS-PAGE | |
A DNA sequence encoding SARS-CoV-2 S1-NTD was expressed in mammalian system with 6*His tag at the N-terminus. |
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