You have no items in your shopping cart.
Cart summary
Item 1 of 6
Item 1 of 6
SARS-CoV-2 (COVID-19) S1 Protein, mFc Tag
Catalog Number: orb640170
| Catalog Number | orb640170 |
|---|---|
| Category | Proteins |
| Description | It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
| Target | Spike S1 |
| Conjugation | Unconjugated |
| Tag | C-mFc(mIgG2a) |
| Reactivity | Virus |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| Protein Sequence | QHD43416.1 |
| MW | 101.9 kDa |
| Application notes | This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 101.9 kDa. The protein migrates as 115-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | SARS-CoV-2 |
| Expression Region | Val 16 - Arg 685 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | QHD43416.1 |
| Expiration Date | 6 months from date of receipt. |
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of of SARS-CoV-2 (COVID-19) S1 protein.
SDS-page analysis of SARS-CoV-2 (COVID-19) S1 protein.
Flow cytometric analysis of Vero E6 cells using SARS-CoV-2 (COVID-19) S1 protein.
Flow cytometric analysis of Vero E6 cells using SARS-CoV-2 (COVID-19) S1 protein.
Flow cytometric analysis of Vero E6 cells using SARS-CoV-2 (COVID-19) S1 protein.
SARS-CoV-2 (COVID-19) S1 protein.
- Item 1 of 1
SARS-CoV-2 (2019-nCoV) S1 protein NTD, mFc Tag [orb689471]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 59.1 kDa after removal of the signal peptide.The apparent molecular mass of S1-NTD-mFc is approximately 70-100 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg 
Recombinant COVID-19/SARS-CoV-2 Spike RBD protein, mFc Tag [orb1141464]
ELISA, WB
≥95% as determined by SDS-PAGE
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) was fused a mFc tag at N terminus was expressed from Mammalian cells can bind with ACE2.
100 μg, 1 mg
