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Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB - 1:1,000 - 1:10,000; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. |
| Reactivity | Bovine, Human, Mouse |
| Predicted Reactivity | Canine, Drosophila, Equine, Feline, Gallus, Guinea pig, Porcine, Rabbit, Rat, Sheep, Xenopus |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1 and 50 |
| Target | Ubiquitin |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of Ubiquitin by western blotting.Samples: A) Ubiquitination assay, B) Purified ubiquitin. Antibody: Affinity purified rabbit anti-Ubiquitin antibody (orb1530366) used at 0.4 µg/ml.

Detection of human and mouse Ubiquitin by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-Ubiquitin used at a dilution of 1:1, 000 (1µg/ml).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_003324.1 |
|---|
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Protocol Information
Rabbit Ubiquitin Antibody (orb1530366)
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