LC3 antibody

SKU: orb33328

Description

LC3 antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-P, WB
• Dilution range: IF/ICC: 1:100-800, IHC-P: 1:100-800, WB: 1:200-2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LC3. Please contact us for the exact immunogen sequence. The peptide is available as orb12928.
• Target: LC3
• Molecular Weight: 14 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti ATG8F antibody, anti Autophagy-related protein LC3 B antibody, anti Autophagy-related ubiquitin-like modifier LC3 B antibody, anti LC3 antibody, anti LC 3 antibody, anti LC3A antibody, anti LC3B antibody, anti LC3II antibody, anti MAP1 light chain 3 like protein 2 antibody, anti MAP1 light chain 3-like protein 2 antibody, anti MAP1A/1BLC3 antibody, anti MAP1A/MAP1B LC3 B antibody, anti MAP1A/MAP1B light chain 3 B antibody, anti MAP1ALC3 antibody, anti MAP1LC3B a antibody, anti Map1lc3b antibody, anti Microtubule associated protein 1 light chain 3 beta antibody, anti Microtubule-associated protein 1 light chain 3 beta antibody, anti Microtubule-associated proteins 1A/1B light chain 3B antibody

IHC-P image of rat prostate tissue using LC3 antibody (dilution of primary antibody at 2.5 ug/ml)

Immunohistochemical staining of mouse heart tissue using LC3 antibody (dilution of primary antibody - 2.5 ug/ml)

Immunohistochemical staining of rat spinal cord tissue using anti- LC3 (dilution of primary antibody - 2.5 ug/ml)

IHC-P staining of rat spinal cord tissue using LC3 antibody (dilution at 2.5 ug/ml)

Immunofluorescence image of rat spinal cord tissue using anti- LC3 (dilution at 2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat brain tissue using LC3 antibody (primary antibody at 2.5 ug/ml)

WB analysis of U251 cells (lane 1), 293T cells (lane 2), mouse brain (lane 3), rat spinal cord (lane 4), rat heart (lane 5), mouse uterus (lane 6), rat bladder (lane 7) using LC3 antibody (1 ug/ml)

Immunohistochemical staining of paraffin embedded rat prostate tissue using LC3 antibody (primary antibody at 2.5 ug/ml)

IF image of rat thymus tissue using LC3 antibody (primary antibody at 2.5 ug/ml)

IHC-P image of rat spinal cord tissue using LC3 antibody (dilution of primary antibody at 2.5 ug/ml)

Wesstern blot analysis of U251 cells (lane 1), 293T cells (lane 2), mouse brain (lane 3), rat spinal cord (lane 4), rat heart (lane 5), mouse uterus (lane 6), rat bladder (lane 7) using LC3 antibody (1 ug/ml)

IHC-P staining of rat brain tissue using anti- LC3 (dilution at 2.5 ug/ml)

IF analysis of rat thymus tissue using LC3 antibody (dilution of primary antibody at 2.5 ug/ml)

Immunohistochemical staining of rat spinal cord tissue using anti- LC3 (dilution of primary antibody - 2.5 ug/ml)

Immunofluorescence analysis of rat spinal cord tissue using LC3 antibody (dilution of primary antibody - 2.5 ug/ml)

Quick Database Links

Gene Symbol

LC3

UniProt

Q9GZQ8

NCBI Gene ID

81631

References

Masoumeh Azimirad, Maryam Noori, Sahar Amirkamali, Gelareh Nasiri, Hamid Asadzadeh Aghdaei, Abbas Yadegar, Daniel J. Klionsky, Mohammad Reza Zali Clostridioides difficile PCR ribotypes 001 and 084 can trigger autophagy process in human intestinal Caco-2 cells Microb Pathog, 185, 106450 (2023)

PubMed: 37979713

Applications

IF

Reactivity

Human