PGP9.5 antibody

• Catalog Number: orb6713
• Category: Antibodies
• Description: PGP9.5 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PGP9.5. Please contact us for the exact immunogen sequence. The peptide is available as orb12924.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: IF/ICC: 1:100-500, IHC-P: 1:100-500, WB: 1:200-1000
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 25 kDa
• Target: PGP9.5
• Entrez: 7345
• UniProt ID: P09936, Q00981, Q9R0P9
• RRID: AB_10940692
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-Epididymis luminal protein 117 antibody, anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ayantika Kundu,Pratiti Ghosh, Biswadev Bishayi Vitexin along with verapamil downregulates efflux pump P-glycoprotein in macrophages and potentiate M1 to M2 switching via TLR4-NF-κB-TNFR3 pathway in Lipopolysaccharide treated mice Immunobiology, 229(1), 152767 (2024)

PubMed: 38103391

Applications

WB

Reactivity

Mouse

Ayantika Kundu 1, Pratiti Ghosh 2, Biswadev Bishayi Verapamil and tangeretin enhances the M1 macrophages to M2 type in lipopolysaccharide-treated mice and inhibits the P-glycoprotein expression by downregulating STAT1/STAT3 and upregulating SOCS3 Int Immunopharmacol, (2024)

PubMed: 38678669

Applications

WB

Reactivity

Mouse

WB analysis of mouse brain (lane 1), rat liver (lane 2), mouse spinal cord (lane 3), mouse heart (lane 4), mouse colon (lane 5), human breast cancer (lane 6) using PGP9.5 antibody (1 ug/ml)

Western blot analysis of mouse brain (lane 1), rat liver (lane 2), mouse spinal cord (lane 3), mouse heart (lane 4), mouse colon (lane 5), human breast cancer (lane 6) using PGP9.5 antibody (1 ug/ml)

Immunohistochemical staining of paraffin embedded rat brain tissue using PGP 9.5 antibody (primary antibody at 2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rabbit liver tissue using anti-PGP 9.5 (primary antibody at 2.5 ug/ml)

IHC-P image of rat brain tissue using PGP 9.5 antibody (dilution of primary antibody at 2.5 ug/ml)

IHC-P staining of rabbit liver tissue using anti-PGP 9.5 (dilution at 2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat brain tissue using PGP 9.5 antibody (primary antibody at 2.5 ug/ml)

Immunohistochemical staining of rabbit liver tissue using PGP 9.5 antibody (dilution of primary antibody - 2.5 ug/ml)

IF image of rat brain tissue using anti-PGP 9.5 (primary antibody at 2.5 ug/ml)

IF image of rabbit liver tissue using PGP 9.5 antibody (primary antibody at 2.5 ug/ml)

Immunofluorescence image of rabbit liver tissue using anti-PGP 9.5 (dilution at 2.5 ug/ml)

IF analysis of rat brain tissue using PGP 9.5 antibody (dilution of primary antibody at 2.5 ug/ml)