Rabbit anti-beta Catenin Antibody

• Catalog Number: orb1527387
• Category: Antibodies
• Description: beta Catenin Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 731 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: beta Catenin
• UniProt ID: P35222
• NCBI: NP_001895.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: armadillo; MRD19; EVR7; CTNNB; catenin beta-1; Bet
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse beta Catenin by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-beta Catenin antibody orb1527387 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human beta Catenin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-beta Catenin antibody (orb1527387) used for IP at 5 µg/mg lysate.

Detection of human beta Catenin by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-beta Catenin (Cat. No. orb1527387) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Localization of beta Catenin Binding Sites by ChIP-sequencing. Chromatin from SW837 cell line was immunoprecipitated with anti-beta Catenin antibody orb1527387 and analyzed by DNA sequencing. The figure illustrates the peak distribution of beta Catenin binding within a 250 Kb region of chromosome 20 as detected using anti-beta Catenin orb1527387.