Perilipin Antibody

• Catalog Number: orb1247118
• Category: Antibodies
• Description: Perilipin Antibody
• Target: PLIN
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-PREKPKRRVSDS
• UniProt ID: O60240
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Adipose lysates (calculated MW of 56.0kDa according to NP_002657.2). Recommended concentration: 0.01 ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 2ug/ml.This product is not suitable for IF but the related product EB07728 is tested in this application.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PLIN, perilipin, PERI, lipid droplet-associated pr
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_002657.2

orb1247118 (0.01 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247118 (2 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.