Perilipin A Rabbit Polyclonal Antibody

SKU: orb5717

Description

Perilipin A Rabbit Polyclonal Antibody

Research Area

Cardiovascular Research, Cell Biology, Lipid Metabolism, Lipids and Lipoproteins Metabolism, Metabolism

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Perilipin-1 (85-180/522aa)
• Target: PLIN1
• Molecular Weight: 57 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lipid droplet associated protein; Lipid droplet-associated protein; PERI; Perilipin; Perilipin-1; Perilipin 1; PerilipinA; Perilipin-A; PLIN; PLIN1; PLIN1_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat ovary), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Perilipin A) Polyclonal Antibody, Unconjugated (orb5717) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Sample: MCF-7 Cell (Human) Lysate at 30 ug, Primary: Anti-Perilipin A at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Sample: Parotid gland (Mouse) Lysate at 40 ug, Primary: Anti-Perilipin A (orb5717) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Sample: Thymus (Mouse) Lysate at 40 ug, Primary: Anti-Perilipin A (orb5717) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Quick Database Links

Gene Symbol

PLIN1

UniProt

O60240

Merve Ercin et al. Exploring the role of cellular plasticity in metabolic dysfunction-associated steatosis and related molecular mechanisms J Transl Med, (2025)

PubMed: 41233849