Perilipin A+B Rabbit Polyclonal Antibody

• Catalog Number: orb100966
• Category: Antibodies
• Description: Perilipin A+B Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Gallus, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Perilipin A+B Mixed peptides
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54-56 kDa
• Target: PLIN1
• UniProt ID: O60240
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Lipid droplet associated protein; PERIA; PERIB; Pe
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: MCF7 (blue). Primary Antibody: Rabbit Anti-Perilipin A+B/AF647 Conjugated antibody, Dilution: 5 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.

Blank control: U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Perilipin A+B antibody (orb100966), Dilution: 0.2 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Sample: HepG2 Cell (Human) Lysate at 30 ug, Primary: Anti-Perilipin A+B (orb100966) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54-56kD, Observed band size: 50kD.

Sample: Stomach (Mouse) Lysate at 40 ug, Primary: Anti-Perilipin A+B (orb100966) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54-56 kD, Observed band size: 56 kD.

Immunostaining of perilipin-positive adipocytes (red) using Rabbit Anti-Perilipin A+B Polyclonal Antibody (orb100966) in bone marrow of IL-7cre Rosa26-EYFP mice with DAPI (blue) nuclear counterstain.