Perilipin 1 Rabbit Polyclonal Antibody

• Catalog Number: orb184407
• Category: Antibodies
• Description: Perilipin 1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Human, Porcine, Sheep
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Perilipin-1 (331-430/522aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=5μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 57 kDa
• Target: PLIN1
• UniProt ID: O60240
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Lipid droplet associated protein; Lipid droplet-as
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): RSC96 Cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Perilipin 1 antibody (orb184407), dilution: 5 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-Perilipin 1 (orb184407) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.