CD274 Antibody

SKU: orb1671421

Description

CD274 Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure Recombinant Human PD-L1 Fc. Anti-Human PD-L1 Fc-specific antibody was purified by affinity chromatography employing an immobilized Human PD-L1 Fc matrix.
• Target: CD274
• Purification: PD-L1 Fc-specific antibody was purified by affinity chromatography employing an immobilized Human PD-L1 Fc matrix
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Programmed Death Ligand 1, Programmed cell death 1 Ligand 1 (PDCD1L1), PD-1, B7-H1 (B7 homolog 1), CD274, SLEB2, SLE1

To detect Human PD-L1 Fc by sandwich ELISA (using 100 μl/well), a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PD-L1 Fc as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PD-L1 Fc.

To detect Human PD-L1 Fc by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human PD-L1 Fc is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

CD274

UniProt

Q9NZQ7

RefSeq:Q9NZQ7