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Product Overview
Product Name CD274 antibody
Catalog Number orb10162
Species/HostRabbit
ReactivityHuman, Mouse, Rat
ConjugationUnconjugated
Tested applicationsIHC-P, P-ELISA, WB
Immunogen KLH conjugated synthetic peptide derived from human CD274. Please contact us for the exact immunogen sequence. The peptide is available as orb374702.
Target CD274
Alternative Names
Product Properties
Form/Appearance 10 mM PBS, 0.02% sodium azide
Concentration - 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Polyclonal antibodies are purified by peptide affinity chromatography
MW 33 kDa
Uniprot ID Q9EP73 (Mouse)
Entrez 29126
Product Description

The CD274 antibody is commonly known as PD-L1. B7-H1 is the target , it is a member of the B7 family, and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. CD274 is also Involved in the signaling , it also helps in the production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Application Notes
Dilution Range IHC-P: 1:50-300 (based on 0.5mg/ml), WB: 1:100-500
Validation Images
Immunohistochemical staining of paraffin embedded human breast cancer tissue using CD274 antibody (2.5ug/ml)
Immunohistochemical staining of paraffin embedded human breast cancer tissue using CD274 antibody (2.5ug/ml)
Immunohistochemical staining of human breast cancer tissue using CD274 antibody (2.5ug/ml)
Immunohistochemical staining of human breast cancer tissue using CD274 antibody (2.5ug/ml)
Western blot analysis of mouse heart, rat lung, rat spleen, A549, human breast cancer, human lung cancer (Lane 1 to Lane 6) using CD274 antibody (1 ug/ml)
Western blot analysis of mouse heart, rat lung, rat spleen, A549, human breast cancer, human lung cancer (Lane 1 to Lane 6) using CD274 antibody (1 ug/ml)
Western blot analysis of mouse heart, rat lung, rat spleen, A549, human breast cancer, human lung cancer (Lane 1 to Lane 6) using CD274 antibody (1 ug/ml)
Western blot analysis of mouse heart, rat lung, rat spleen, A549, human breast cancer, human lung cancer (Lane 1 to Lane 6) using CD274 antibody (1 ug/ml)
WB analysis of  mouse heart (lane 1), rat muscle  (lane 2), rat lung (lane 3), rat thymus (lane 4), rat lymph node (lane 5)  using  CD274 antibody  (2.5 ug/ml)
WB analysis of mouse heart (lane 1), rat muscle (lane 2), rat lung (lane 3), rat thymus (lane 4), rat lymph node (lane 5) using CD274 antibody (2.5 ug/ml)
Immunohistochemical staining of rat heart tissue using CD274 antibody (2.5 ug/ml)
Immunohistochemical staining of rat heart tissue using CD274 antibody (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat heart tissue using CD274 antibody (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat heart tissue using CD274 antibody (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat thymus tissue using anti-CD274 (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat thymus tissue using anti-CD274 (2.5 ug/ml)
IHC-P image of rat heart tissue using CD274 antibody (2.5 ug/ml)
IHC-P image of rat heart tissue using CD274 antibody (2.5 ug/ml)
IHC-P image of mouse spleen tissue using anti-CD274 (2.5 ug/ml)
IHC-P image of mouse spleen tissue using anti-CD274 (2.5 ug/ml)
IHC-P staining of rat thymus tissue using anti-CD274 (2.5 ug/ml)
IHC-P staining of rat thymus tissue using anti-CD274 (2.5 ug/ml)
IHC-P staining of rat thymus tissue using anti-CD274 (2.5 ug/ml)
IHC-P staining of rat thymus tissue using anti-CD274 (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat thymus tissue using anti-CD274 (2.5 ug/ml)
Immunohistochemical staining of paraffin embedded rat thymus tissue using anti-CD274 (2.5 ug/ml)
Western blot analysis of  mouse heart (lane 1), rat muscle  (lane 2), rat lung (lane 3), rat thymus (lane 4), rat lymph node (lane 5)  using  CD274 antibody  (2.5 ug/ml)
Western blot analysis of mouse heart (lane 1), rat muscle (lane 2), rat lung (lane 3), rat thymus (lane 4), rat lymph node (lane 5) using CD274 antibody (2.5 ug/ml)
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