CD274 antibody

• Catalog Number: orb10162
• Category: Antibodies
• Description: CD274 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD274. Please contact us for the exact immunogen sequence. The peptide is available as orb374702.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: IHC-P: 1:50-300 (based on 0.5mg/ml), WB: 1:100-500
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 33 kDa
• Target: CD274
• Entrez: 29126
• UniProt ID: Q9EP73
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: Anti-CD274 antibody, anti-Programmed cell death 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Hubert, Pascale et al. Extracellular HMGB1 blockade inhibits tumor growth through profoundly remodeling immune microenvironment and enhances checkpoint inhibitor-based immunotherapy J Immunother Cancer, 9, (2021)

PubMed: 33712445

Western blot analysis of mouse heart (lane 1), rat muscle (lane 2), rat lung (lane 3), rat thymus (lane 4), rat lymph node (lane 5) using CD274 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat thymus tissue using anti-CD274 (2.5 ug/ml)

IHC-P staining of rat thymus tissue using anti-CD274 (2.5 ug/ml)

IHC-P staining of rat thymus tissue using anti-CD274 (2.5 ug/ml)

IHC-P image of mouse spleen tissue using anti-CD274 (2.5 ug/ml)

IHC-P image of rat heart tissue using CD274 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat thymus tissue using anti-CD274 (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat heart tissue using CD274 antibody (2.5 ug/ml)

Immunohistochemical staining of rat heart tissue using CD274 antibody (2.5 ug/ml)

WB analysis of mouse heart (lane 1), rat muscle (lane 2), rat lung (lane 3), rat thymus (lane 4), rat lymph node (lane 5) using CD274 antibody (2.5 ug/ml)

Western blot analysis of mouse heart, rat lung, rat spleen, A549, human breast cancer, human lung cancer (Lane 1 to Lane 6) using CD274 antibody (1 ug/ml)

Western blot analysis of mouse heart, rat lung, rat spleen, A549, human breast cancer, human lung cancer (Lane 1 to Lane 6) using CD274 antibody (1 ug/ml)

Immunohistochemical staining of human breast cancer tissue using CD274 antibody (2.5ug/ml)

Immunohistochemical staining of paraffin embedded human breast cancer tissue using CD274 antibody (2.5ug/ml)