CD274 Antibody (Biotin)

SKU: orb751161

Description

Mouse monoclonal antibody against CD274 conjugated to Biotin

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: Flow Cytometry (1-2ug/million cells); ,Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human, Mouse
• Application Notes:
  Positive Control: Jurkat, RAW, HEK293 or HepG2 cells. Human lung SqCC, cervical or breast carcinoma.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: PDL1/2746
• Immunogen: Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)
• Target: CD274
• Source/Expression System: Bioreactor
• Molecular Weight: 37-50kDa
• Purification: Protein A
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-B7 homolog 1 antibody, anti-B7-H1 antibody, anti-CD274 antibody, anti-PD-L1 antibody, anti-PDCD1 ligand 1 antibody, anti-PDCD1L1 antibody, anti-PDCD1LG1 antibody, anti-Programmed cell death 1 ligand 1 antibody

Quick Database Links

Gene Symbol

CD274

UniProt

Q9NZQ7

NCBI Gene ID

29126