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Catalog Number | orb1249007 |
---|---|
Category | Antibodies |
Description | PCBP1 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Canine, Mouse |
Reactivity | Human, Rat |
Immunogen | The immunogen for this antibody is: C-SIQGQHTISPLD |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 38kDa band observed in Human Peripheral Blood Nucleocytes lysates (calculated MW of 37.5kDa according to NP_006187.2). The 38kDa was also observed in Rat Spleen while a 37kDa band was observed in Rat Thymus. Recommended concentration: 0.1-0.3ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | PCBP1 |
UniProt ID | Q15365 |
NCBI | NP_006187.2 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | PCBP1, poly(rC) binding protein 1, HNRPE1, HNRPX, Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 38kDa band observed in Human Peripheral Blood Nucleocytes lysates (calculated MW of 37.5kDa according to NP_006187.2). The 38kDa was also observed in Rat Spleen while a 37kDa band was observed in Rat Thymus. Recommended concentration: 0.1-0.3ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. |
Expiration Date | 12 months from date of receipt. |
orb1249007 (0.1 ug/ml) staining of PBMC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb1249007 (0.1 ug/ml) staining of Rat Spleen (A) and Thymus (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Animal, Bovine, Canine, Guinea pig, Human, Mouse, Rabbit, Rat | |
Canine, Equine, Guinea pig, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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