PCBP1 (aa223-234) Antibody

• Catalog Number: orb125087
• Category: Antibodies
• Description: Goat Polyclonal to PCBP1. NP_006187.2, PMID: 19211566 and GeneID: 5093.
• Target: PCBP1 (aa223-234)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SIQGQHTISPLD
• MW: 37.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml
• Application notes: WB: Approx 38kDa band observed in Human Peripheral Blood Nucleocytes lysates (calculated MW of 37.5kDa according to NP_006187.2). The 38kDa was also observed in Rat Spleen while a 37kDa band was observed in Rat Thymus. Recommended concentration: 0.1-0.3µg/ml. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PCBP1 antibody, anti poly(rC) binding protein
• Note: For research use only
• Entrez: 5093
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PBMC lysate using PCBP1 antibody

Western blot analysis of Rat Spleen (Lane 1) and Thymus (Lane 2) lysate using PCBP1 antibody