PCBP1 Antibody

• Catalog Number: orb1249006
• Category: Antibodies
• Description: PCBP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-ADLAKLNQVARQQSH
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of cell line HeLa and in Human Placenta lysates (calculated MW of 37.5kDa according to NP_006187.2). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Lung (respiratory epithelium). Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PCBP1
• UniProt ID: Q15365
• NCBI: CAA55016.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PCBP1, poly(rC) binding protein 1, HNRPE1, HNRPX,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in lysates of cell line HeLa and in Human Placenta lysates (calculated MW of 37.5kDa according to NP_006187.2). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Lung (respiratory epithelium). Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249006 (0.01 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249006 (5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.