OCLN Rabbit Polyclonal Antibody

SKU: orb11181

Description

OCLN Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2µg/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse OCLN (431-522/522aa)
• Target: OCLN
• Molecular Weight: 57 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

OCLN_HUMAN; Occludin; Ocl; BLCPMG; PPP1R115; PTORCH1; Tight junction protein occludin;

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-OCLN antibody (orb11181), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: MCF-7 (Human) Cell Lysate at 30 ug, Lane 3: A431 (Human) Cell Lysate at 30 ug, Lane 4: HepG2 (Human) Cell Lysate at 30 ug, Lane 5: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-OCLN (orb11181) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65/55/38/28 kD, Observed band size: 65/38/28 kD.

The blue histogram is unstained cells (293FT cells). The Wathet Blue histogram is cells stained with secondary antibody alone. The Orange histogram is cells stained with rabbit IgG isotype control antibody, plus secondary antibody. The green histogram is cells stained with Rabbit Anti-OCLN antibody (orb11181) plus secondary antibody (Concentraion 1:20).

Quick Database Links

Gene Symbol

OCLN

UniProt

Q61146

References

Liu, Haitao et al. Neutrophil elastase contributes to the pathological vascular permeability characteristic of diabetic retinopathy Diabetologia, (2019)

PubMed: 31612267

Applications

WB

Reactivity

Human

Ma, Xiaojun et al. Lidocaine attenuates hypoxia/reoxygenation?induced inflammation, apoptosis and ferroptosis in lung epithelial cells by regulating the p38 MAPK pathway Mol Med Rep, 25, 150 (2022)

PubMed: 35244190

Applications

WB

Reactivity

Human