Occludin Rabbit Polyclonal Antibody

• Catalog Number: orb185667
• Category: Antibodies
• Description: Occludin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Occludin (151-250/522aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=2µg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 59 kDa
• Target: OCLN
• UniProt ID: Q16625
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Elham Zahedi 1 2, Fatemeh Mokhtari Naseri 3, Elham Zamani 4, Marjan Nikbakhtzadeh 5, Tayebeh Rastegar 6, Ashkan Sanaeirad 7, Seyed Shahabeddin Sadr Ginger Extract Improves Cognitive Dysfunction via Modulation of Gut Microbiota-Derived Short-Chain Fatty Acids in D-Galactose/Ovariectomy-Induced Alzheimer-Like Disease Mol Neurobiol, (2024)

PubMed: 39505806

Blank control (blue): 293T (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Occludin/FITC Conjugated antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Occludin antibody (orb185667), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-Occludin antibody (orb185667), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Occludin (orb185667) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65/55/38/28 kD, Observed band size: 62/52/40/25 kD.