OCLN/occludin Antibody

• Catalog Number: orb1294379
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Occludin
• Target: OCLN / occludin
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human Occludin
• Tested applications: IF, IHC-Fr, WB
• Dilution range: Western Blot 1:500-1:1000 Immunofluorescence 1:200–1:400 Immunohistochemistry (Frozen section)1:100
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

All lanes: Anti-Occludin antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294379 overnight at 4°C.

Occludin antibody by Frozen-section; Sample: Rat brain. Green: Occludin antibody diluted at 1:100. Blue: DAPI.

All lanes: Anti-Occludin antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294379 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294379 anti-Occludin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.