MGMT Antibody

SKU: orb1826177

Description

Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross-links at the O6- alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6- position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the MGMT antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG, kappa
• Clone No.: MGMT/8364R
• Immunogen: A recombinant partial protein (within amino acids 1-100) from the human protein was used as the immunogen for the MGMT antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human prostate tissue with MGMT antibody (clone MGMT/8364R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with MGMT antibody (clone MGMT/8364R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC testing of FFPE human brain tissue with MGMT antibody (clone MGMT/8364R) at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free MGMT antibody (clone MGMT/8364R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P16455