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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | IF - 1:25, WB - 1:1000, IHC - 1:100, IHC-P - 1:25 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | B000EV44X4X6 |
| Immunogen | Purified His-tagged MGMT protein was used to produced this monoclonal antibody. |
| Target | MGMT |
| Molecular Weight | 21646 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase, MGMT
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MGMT Antibody western blot analysis in MCF-7 cell line lysates (35μg/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).
Immunohistochemical analysis of paraffin-embedded Human Prostate cancer section using Pink1. Diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. small intestine section using MGMT Antibody. Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of Hela cells stained with MGMT Antibody. Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Quick Database Links
Gene Symbol
MGMT
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
MGMT Antibody (orb1927312)
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