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Catalog Number | orb334008 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to MGMT |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1A11F8 |
Tested applications | ELISA, FC, ICC, IHC, WB |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Purified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli. |
Concentration | 1mg/ml |
Dilution range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; RCM: 1/200 - 1/400; ELISA: 1/10000 |
Conjugation | Unconjugated |
MW | 21.6kDa |
Entrez | 4255 |
Storage | 4°C; -20°C for long term storage. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
Alternative names | EC 2.1.1.63 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Rodriguez, Fausto J. et al. MGMT immunohistochemical expression and promoter methylation in human glioblastoma Appl Immunohistochem Mol Morphol, 16, 59-65 (2008)
Immunofluorescence analysis of Hela cells using MGMT antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rectum cancer tissues using MGMT antibody
Western blot analysis of human recombinant protein using MGMT antibody
Western blot analysis of HEK293 using MGMT antibody
Western blot analysis of MCF-7 (Lane1), Jurkat (Lane2), HepG2 (Lane3), and PC-3 (Lane4) cell lysate using MGMT antibody
ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Flow cytometric analysis of Hela cells using MGMT antibody
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