MDA5 Antibody

• Catalog Number: orb1247277
• Category: Antibodies
• Description: MDA5 Antibody
• Target: IFIH1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Monkey
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: SNGYSTDENFRYL-C
• UniProt ID: Q9BYX4
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:This antibody has been successfully used in Western blot on African Green Monkey: Sato S et al, Arthritis Rheum. 2009 Jul;60(7):2193-200. PMID: 19565506.Immunohistochemistry:In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IFIH1, MDA5, MDA-5, Hlcd, MGC133047, IDDM19, melan
• Note: For research use only
• NCBI: NP_071451.2

orb1247277 (10 ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.