IFIH1/MDA5 Antibody

• Catalog Number: orb1537746
• Category: Antibodies
• Description: IFIH1/MDA5 Antibody
• Target: IFIH1 / MDA5
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Purification: Peptide Column Affinity Chromatography
• Immunogen: A 16 AA synthetic peptide near the C-Terminus of human MDA5. The immunogen is located within the last 50 AA of MDA5.
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: ELISA, IF (20 µg/ml), IHC (5 µg/ml), IHC-P (5 µg/ml), WB (1 - 4 µg/ml)
• Application notes: Further information: MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 113 kDa Observed: 115 kDa
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IFIH1, Helicase with 2 CARD domains, MDA-5, MDA5,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/ml.

Western blot analysis of Anti-MDA5 antibody (orb1537746, 0.4 µg/ml; 15 µg of lysate per lane). Lane 1: Daudi cell line. Lane 2: HeLa cell line. Antibody produced a predominant band at ~117kDA in both cell lines.