MDA5 Antibody

SKU: orb1238796

Description

MDA5 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Application Notes:
  MDA5 antibody can be used for detection of MDA5 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: MDA5 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human MDA5.The immunogen is located within the last 50 amino acids of MDA5.
• Target: IFIH1
• Molecular Weight: Predicted: 113 kDa Observed: 115 kDa
• Purification: MDA5 Antibody is affinity chromatography purified via peptide column.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: MDA5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

MDA5 Antibody: Hlcd, MDA5, MDA-5, RLR-2, IDDM19, RH116, Interferon-induced helicase C domain-containing protein 1, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen

Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 µg/mL.

Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 2.5 µg/ml.

Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.

Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 µg/mL.

Quick Database Links

Gene Symbol

IFIH1

UniProt

Q9BYX4

RefSeq (Protein):NP_071451