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| Catalog Number | orb18521 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to IFIH1 |
| Target | MDA5 / IFIH1 |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human, Monkey |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | SNGYSTDENFRYL |
| RRID | AB_10751235 |
| MW | 117 |
| Tested applications | ELISA, FC, IHC, WB |
| Dilution range | ELISA: 1:32000, IHC-P: 10ug/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration, 10ug/ml.WB: No signal obtained yet but low background observed in Human Placenta lysate at up to 1 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti IFIH1 antibody, anti MDA5 antibody, anti MDA- Read more... |
| Research Area | Epigenetics, Immunology, Infectious Diseases |
| Note | For research use only |
| Entrez | 64135 |
| Expiration Date | 12 months from date of receipt. |

orb18521 (10ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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