MAX Antibody

• Catalog Number: orb1247944
• Category: Antibodies
• Description: MAX Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EEPQSRKKLRMEAS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 22kDa band observed in lysates of cell line Jurkat (calculated MW of 18.3kDa according to NP_002373.3). Recommended concentration: 0.01-0.03ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 10ug/ml.Immunofluorescence: Expression of the protein seen in the cytoplasm and nuclei of A431 and U251 cells. Recommended concentration: 10ug/ml. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MAX
• UniProt ID: P61244
• NCBI: NP_002373.3, NP_660087.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: MAX, MAX protein, MGC10775, MGC11225, MGC18164, MG
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 22kDa band observed in lysates of cell line Jurkat (calculated MW of 18.3kDa according to NP_002373.3). Recommended concentration: 0.01-0.03ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 10ug/ml.Immunofluorescence: Expression of the protein seen in the cytoplasm and nuclei of A431 and U251 cells. Recommended concentration: 10ug/ml. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

orb1247944 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and nuclear staining.

orb1247944 (0.01 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247944 (10 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247944 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and nuclear staining.